Skip Navigation



Journal of Tropical Pediatrics Advance Access published online on May 12, 2007
Journal of Tropical Pediatrics, doi:10.1093/tropej/fmm021
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
53/4/264    most recent
fmm021v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nguyen, T. A.
Right arrow Articles by Ushijima, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nguyen, T. A.
Right arrow Articles by Ushijima, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2007]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Evaluation of Immunochromatography Tests for Detection of Rotavirus and Norovirus among Vietnamese Children with Acute Gastroenteritis and the Emergence of a Novel Norovirus GII.4 Variant

Tuan Anh Nguyena,b, Pattara Khamrina, Sayaka Takanashia, Phuc Le Hoangb, Le Duc Phamb, Kim Trong Hoangb, Kenji Satouc, Yuichi Masuokac, Shoko Okitsua and Hiroshi Ushijimaa

aDepartment of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
bChildren's Hospital 1, Ho Chi Minh City, Vietnam
cImmuno-Probe Co., Ltd, Kamagata, Ranzan-machi, Saitama, Japan

Correspondence: Hiroshi Ushijima, Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033, Japan. Tel/Fax: 81-3-5841-3629. E-mail < ushijima{at}m.u-tokyo.ac.jp>.


  Abstract

A prospective study was conducted to evaluate two immunochromatography (ICG) tests for detection of group A rotavirus and norovirus GII, the commercial Dipstick ‘Eiken’ Rota kit (SA Scientific, USA) and the NV IC-1 stick (Immuno-Probe, Japan). Polymerase chain reaction (PCR) with specific primer pairs (Beg9 and VP7-1’, for group A rotavirus; COG2F and G2SKR, for norovirus GII) was used as the reference method. The results of ICG tests were compared with those of reference method. The sensitivity, specificity and agreement between ICG tests and PCR were 87.8%, 93.3% and 89.4%, respectively, for rotavirus ICG test; and 73.7%, 100% and 95.2%, respectively, for norovirus ICG test. The immunochromatography assay for norovirus used in this study could detect not only common noroviruses, but also a novel norovirus GII.4 variant, which emerged in Ho Chi Minh City in 2006. Immunochromatography tests are easy, rapid and useful assays for detection of rotavirus and norovirus among pediatric patients with acute gastroenteritis in Vietnam.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Trop PediatrHome page
T. A. Nguyen, L. Hoang, L. D. Pham, K. T. Hoang, S. Okitsu, M. Mizuguchi, and H. Ushijima
Norovirus and Sapovirus Infections among Children with Acute Gastroenteritis in Ho Chi Minh City during 2005-2006
J Trop Pediatr, April 1, 2008; 54(2): 102 - 113.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.