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Journal of Tropical Pediatrics Advance Access originally published online on January 26, 2005
Journal of Tropical Pediatrics 2005 51(2):87-92; doi:10.1093/tropej/fmh084
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© Oxford University Press 2005; all rights reserved

Original Papers

Bacterial Contamination and Nutrient Content of Home-Prepared Milk Feeding Bottles of Infants Attending a Public Outpatient Clinic

Tania Beninga Morais1, Dirce Maria Sigulem2, Hélcio de Sousa Maranhão3 and Mauro Batista de Morais4

1 Department of Pediatrics, Federal University of São Paulo Medical School, São Paulo, Brazil, 2 Department of Pediatrics, Federal University of São Paulo Medical School, São Paulo, Brazil, 3 Department of Pediatrics, Federal University of Rio Grande do Norte Medical School, Natal, Brazil, 4 Department of Pediatrics, Federal University of São Paulo Medical School, São Paulo, Brazil

Tania B. Morais, R. Araioses, 75 05442–010 São Paulo, SP, Brazil. E-mail <tania.pnut{at}epm.br>.

To evaluate the bacterial contamination and the nutrient content of home-prepared milk feeding bottles, 48 samples of their lacteal contents were collected; of these, 29 samples were cultured and 48 were analyzed to determine their macronutrients composition by chemical analyses. The medians (percentiles 25 and 75) of the counts (bacteria/ml) were mesophilic bacteria, 78 000 (500–1 125 000) and coliforms, 600 (0–44 000). Adding sugar (41/48) and cereals (39/48) to the milk was a common practice. The medians of the macronutrients contents (g/dl or kcal/dl) of the feeding bottles with and without the addition of cereals were, respectively: protein, 2.8/2.4; fat, 1.1/2.2; carbohydrate, 14.5/8.6; energy, 88.7/57.2. Feeding bottles with the addition of cereals had carbohydrate and energy contents significantly higher. Home-prepared milk feeding bottles were heavily contaminated. Most milk feeding bottles met or exceeded the recommended values proposed by European Society of Pediatric Gastroenterology and Nutrition for follow-up formulas, except for fat.


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