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Journal of Tropical Pediatrics 1998 44(2):92-95; doi:10.1093/tropej/44.2.92
© 1998 by Oxford University Press
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Respiratory Syncytial Virus Infection in Infants Hospitalized with Respiratory Illness in Northern Jordan

M. M. M. Meqdam*, M. O. Rawashdeh**, H. Masaadeh*, A. A. Shurman** and N. Abuharfeil*

*Department of Applied Biology, Jordan University of Science and Technology P.O. Box 3030, Irbid 22110, Jordan
**Department of Paediatrics, Princess Rahma Hospital Irbid, Jordan

During the winter seasons of 1993 and 1994, a total of 256 nasopharyngeal aspirates (NPA) from Infants aged less than 1 year old admitted to the pediatric ward of Princess Rahma Hospital, northern Jordan, with bronchlolitis and/or pneumonia, were tested for the presence of respiratory syncytlal virus (RSV) using direct immunofluorescence assay (DFA) and cell culture (CC). Of the 256 specimens, 129(50 per cent) were found positive by both DFA and CC, whereas 24 spedmens (9 per cent) and 16 specimens (6 per cent) were found positive by DFA and CC, respectively. In an evaluation of the collected NPA specimens detected by DFA, a sensitivity of 89 per cent and a specificity of 78 per cent were demonstrated. These data suggest that virus isolation in CC is still important for the diagnosis of RSV, although DFA is a valuable, rapid diagnostic assay.


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