Skip Navigation

Journal of Tropical Pediatrics 1997 43(5):307-310; doi:10.1093/tropej/43.5.307
© 1997 by Oxford University Press
This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Munshi, M. M. H.
Right arrow Articles by Rahaman, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Munshi, M. M. H.
Right arrow Articles by Rahaman, M. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

brief-report

Evaluation of Teknaf Enteric Agar (TEA): A Modified MacConkey's Agar for the Isolation of Shigella dysenteriae Type 1 and Shigella flexneri

M. M. H. Munshi*, M. G. Morshed** {ddagger}, M. Ansaruzzaman*, K. Alam*, A. Kay*, K. M. Sultanul Aziz*** {ddagger} and M. Mujibur Rahaman{dagger} {ddagger}

*International Centre for Diarrhoeal Disease Research Bangladesh (ICDDR, B), GPO Box 128, Dhaka-1000, Bangladesh
**Department of Botany, Institute of Life Science, Jahangirnagar University Savar, Dhaka-1342, Bangladesh
***National Kidney Foundation PO.O. Box 27049, Riyadh, Kingdom of Saudi Arabia
{dagger}Food and Agricultural Organization (FAQ) P.O. Box: 10085, Sariyac Sharkia, Baghdad, Iraq

To develop a better and selective medium for the isolation of Shigella spp., MacConkey's Agar (MAC) was modified by adding potassium tellurite (K2TeO3 at a concentration of 1 µg/ml. The formulation designated Teknaf Enteric Agar (TEA) was studied for the inhibitory effect of potassium tellurite on the growth of different enteric bacteria, and as a medium for isolating Shigella spp. from clinical stool samples (n = 3125). We observed that the growth of E. coli was effectively inhibited on TEA with no effect on the growth of S. dysenteriae type l and S.flexneri. A total of 2019 Shigellae were isolated through the combined use of TEA, MAC, and Salmonella-Shigella Agar (SS). On TEA, 1921 S. dysenteriae type 1 and S.flexneri were isolated as compared to 1765 from the combined use of MAC and SS. A total of 194 of S. dysenteriae type 1 and S. flexneri were exclusively isolated from TEA as compared to 38 which were only made from MAC and SS. We conclude that TEA significantly increased the overall isolation rate of Shigella spp. as compared to the combined use of MAC and SS (P < 0.0001), although it is not suitable for the isolation of S. sonnei.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.