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Journal of Tropical Pediatrics 1997 43(4):208-212; doi:10.1093/tropej/43.4.208
© 1997 by Oxford University Press
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Immunodiagnoses of Community-acquired Pneumonia in Childhood

Henry I. Z. Requejo*, Maria Luiza L. S. Guerre*, Marlina Dos Santos** and Ana Maria Cocozza***

*Instituto Adolfo Lutz, Department of Immunology, Avenida Dr Arnaldo 355 São Paulo, SP 01246–902, Brazil
**Hospital Infantil Cândido Fontoura São Paulo, Brazil
***Hospital Infantil Menino Jesus São Paulo, Brazil

A diagnosis of bacterial pneumonia requires isolation of the pathogen from blood, lung or tracheal aspirate; however, cultures of blood and pleural fluid samples are usually insensitive. Thus, in the majority of patients the etiology is rarely determined. A total of 840 pleural fluid effusion samples from children with clinical and laboratory diagnoses of acute bacterial pneumonia were evaluated by Dot-ELISA. This method was standardized in order to detect polysaccharide capsular bacterial antigen in pleural fluid samples previously treated with 0.1 M EDTA and dotted on nitrocellulose membrane strips. Pneumococcal omniserum and H. influenzae type b antiserum diluted 1:200 were employed for detection of S. pneumoniae and H. influenzae type b antigens, respectively. Dot-ELISA showed relative indices of 0.913 for sensitivity and 0.552 for specificity, and a total positivity of 75.6 per cent, being 53.21 per cent for S. pneumoniae and of 22.38 per cent for H. influenzae.


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